fluoroSENS Steady-State Fluorescence Spectrometers
The fluoroSENS is a modular, computer controlled ultra-high sensitivity fluorimeter for measuring fluorescence excitation and emission spectral from the ultraviolet-near to very near infrared spectral range with single photon counting sensitivity, and a Water Raman signal-to-noise ratio of >3000:1. The performance specifications of the fluoroSENS make it ideally suited for demanding applications in the fields of photo-physics, chemistry, biology, medical science, semiconductor, and environmental etc.
The fluoroSENS brings the ultimate single photon counting sensitivity into a fully computer controlled fluorimeter that is designed for both the demands of research users as well as routine laboratory users. |
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Application Fields:
Biochemistry
Cytotoxin, ion concentration quantitative analysis, cell proliferation, DNA ration, quantitative chemical analysis.
Environmental monitoring
Monitor trace quantities of drug residues, monitor the water quality, pollutant analysis.
Drug Development & pharmacology
Routine analysis, investigation of protein drug, drug interaction in the biological system, quinolone antibiotics, high throughput screening.
Food science & agriculture
Assessment of shelf life& packaging, bacterial growth, quality control, pesticide analysis.
Challenge your limits of Sensitivity
----- single photon counting technology, the best one can achieve.
Single photon counting is a unique technology for the measurement of low level optical radiation. When the light level becomes so low that the output pulses from the photomultiplier are automatically
discrete, then, single photon counting technology can record significantly lower light signals from the background noise, using both impulse amplitude discrimination and digital counting technology. When a photon strikes the photocathode, one photo-electron is emitted from the photocathode with the given production probability defined by the quantum efficiency( QE) pf the photocathode. This photo-electron is multiplied by the photomultiplier to create a group of electrons and is derived from the anode as a current impulse. This pulse is called a single photon pulse. Low light level detection is accomplished by selecting the photon pulses from the noise pulse by using a discriminator to reject the low voltage noise pulses and select only the single photon pulses. As a result single photon counting can achieve detections levels up to 10,000 more sensitive than conventional fluorimeter systems. For example, the measurement of Fluorescein concentrations as low as 50 to 100fM can be routinely measured using the fluoroSENS instrument. This sensitivity allows the spectra of ordinary fluorescence and weak fluorescence signal to be routinely measured.
Often quoted as a measure of the sensitivity performance, the water Raman signal-to-noise ratio is a “standard” by whichy many instruments can be compared. The fluoroSENS is guarantied to achieve a water Raman signal-to-noise greater than 3000:1, which is at least 10 to 100 times more sensitive then regular fluorimeter instruments. Of course the definition and measurement conditions for this measurement are critical for comparison and often manufacturers use non-standard conditions to improve or manipulate the result .
Of course, sensitivity is only part of the story and the fluoroSENS has a photon counting system with a 100M counts per second propriety counter systems which has been optimised of the measurement of both high and low signal rates. In this way the instrument has a superb dynamic range, along with its excellent stray light performance, allows the measurement of intense spectral features beside low intensity ones.
